Feeding Protocol Modulates the Digestive Process in Senegalese Sole (Solea senegalensis) Juveniles

Understanding the daily digestive function could lead to more efficient feeding practices in farmed fish species. In this work, effect of several protocols was assessed on expression genes related circadian system ( arntl1, clock, per1 , and cry1 ), appetite cck nutrients hydrolysis try1, ctrb1, pga, atp4a, cel amy2a peptides transport slc15a1 pH, activity key (trypsin, chymotrypsin, amylase, 4C- 7C-like lipases) absorptive (alkaline phosphatase) enzymes gastrointestinal tract (GIT) Senegalese sole Solea senegalensis ) juveniles. Some tested demonstrated a significant influence entrainment peripheral oscillator. The synchrony some proenzymes with experimental treatments underline role clock regulation these biological functions. Unlike gene wherein protocol occasionally affected temporal organization, factor determinant average production and/or pattern their activities. Daily pH maintained at approximately neutral levels all GIT segments, no pepsin detected. Fish fed single diurnal meal showed lowest for studied enzymes, except suggesting an insufficient utilization ingested nutrients. Higher amylase group, other hand, implies possible activation alternative compensatory metabolic pathways. Overall, our results pointed suitability higher frequencies, especially during dark hours, aquaculture